Enhancing Forest Trees With Genome Editing

Project Title

GRO FORTH: Genomic Resource Optimization for Forest Health

Project Description

This project aims to develop a reference quality genome sequence for Fraser fir trees and tissue specific gene expression information of loblolly pine trees. This information will refine current knowledge of defense gene sequences in these species and help identify sequences for the development of genetically-improved Fraser fir and loblolly pine trees, which will positively impact forest health and Christmas tree production in the southeastern United States and beyond. 

Researchers will also recruit and educate a graduate student to apply molecular technologies for rapid improvements to forest health and productivity. With increasing temperatures driving unprecedented numbers of outbreaks of forest insect pests and diseases, the recruitment and training of well-trained specialists that can develop and implement these technologies is the primary means to combat the inevitable outcomes associated with a changing climate.

Principal Investigators

Justin Whitehill (Director)
Ross Whetten
Jack Wang 

Collaborators

This project unites faculty members from across the Department of Forestry and Environmental Resources who have complimentary strengths in the area of forest genetics and genomics. 

Expected Outcomes

This project will ensure a more robust, efficient and sustainable Christmas tree industry in the southeastern U.S. 

• Development of a reference quality genome sequence for Fraser fir and tissue specific gene expression libraries of loblolly pine to provide a solid foundation to build future research projects upon for years to come.
• Utilization and evaluation of genomic resources to refine knowledge of the conifer defense gene space in Fraser fir and loblolly pine.
• Identification of the major and minor components of loblolly pine and Fraser fir oleoresin. 
• Identification of target gene sequences of oleoresin metabolites to facilitate CRISPR-based genome editing of Fraser fir somatic embryos to regenerate enhanced clonal trees and improve forest health of loblolly pine through tree improvement efforts and the application of genomic tools.
• Training of a graduate student in the modern genomics toolkit to apply to improve forest health and productivity.

About McIntire-Stennis

The McIntire-Stennis program, a unique federal-state partnership, cultivates and delivers forestry and natural resource innovations for a better future. By advancing research and education that increases the understanding of emerging challenges and fosters the development of relevant solutions, the McIntire-Stennis program has ensured healthy resilient forests and communities and an exceptional natural resources workforce since 1962.